The fusicoccin receptor of plants is a member of the 14-3-3 superfamily of eukaryotic regulatory proteins

FEBS Lett. 1994 Sep 26;352(2):163-6. doi: 10.1016/0014-5793(94)00949-x.


The receptor for the wilt-inducing phytotoxin fusicoccin was purified to homogeneity from plasma membranes of Commelina communis as a complex with the radioligand [3H]9'-nor-8'-hydroxyfusicoccin. The preparation consisted of two polypeptides with apparent molecular masses of 30.5 kDa and 31.5 kDa and with isoelectric points of around pH 5.2 and 5.3, respectively. The proteins were N-terminally blocked. Internal amino acid sequences were obtained for both polypeptides of the fusicoccin-binding complex. Sequence information, as well as subsequent immunological analysis, proved that both polypeptides are members of the eukaryotic 14-3-3 family, which comprises structurally conserved regulatory proteins of widespread occurrence and a wide range of functions. 14-3-3 isoform(s) constituting the fusicoccin receptor are distinguishable from other cellular 14-3-3 proteins by their tight association with the plasma membrane. Applying temperature-induced Triton X-114 phase separation experiments, they, as well as the target enzyme of fusicoccin action, the H(+)-ATPase, partitioned into the phospholipid-rich fraction which contains the most hydrophobic proteins. The results discussed herein provide a basis for the elucidation of the molecular mechanism of fusicoccin action.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Amino Acid Sequence
  • Cell Fractionation
  • Cell Membrane / chemistry
  • Glycosides / pharmacology
  • Isoelectric Point
  • Molecular Sequence Data
  • Molecular Weight
  • Plant Proteins*
  • Plants / chemistry*
  • Radioligand Assay
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / isolation & purification
  • Sequence Alignment
  • Sequence Analysis
  • Sequence Homology, Amino Acid
  • Signal Transduction


  • Glycosides
  • Plant Proteins
  • Receptors, Cell Surface
  • fusicoccin receptor protein, plant
  • fusicoccin
  • Adenosine Triphosphatases