Expression of TIMP3 mRNA is elevated in retinas affected by simplex retinitis pigmentosa

FEBS Lett. 1994 Sep 26;352(2):171-4. doi: 10.1016/0014-5793(94)00951-1.


To explore the molecular and cellular mechanisms associated with photoreceptor death in retinitis pigmentosa (RP), we have investigated altered transcriptional activity in RP retinas by a differential cDNA screening approach. We identified a clone (K222) showing over-expression in simplex RP retinas compared with controls. K222 encodes a partial cDNA of the human tissue inhibitor of metalloproteinases-3 (TIMP3) gene, a member of a family of genes implicated in extracellular matrix (ECM) remodelling. Increased expression of TIMP3 in degenerating RP retinas may reflect restructuring of the ECM architecture, and disruption of photoreceptor-matrix interactions could contribute to activation of apoptotic cell death processes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Apoptosis
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22
  • DNA, Complementary / analysis
  • Extracellular Matrix / metabolism
  • Female
  • Gene Expression Regulation*
  • Humans
  • Male
  • Metalloendopeptidases / antagonists & inhibitors
  • Middle Aged
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Retina / metabolism*
  • Retinitis Pigmentosa / metabolism*
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Tissue Inhibitor of Metalloproteinase-3


  • DNA, Complementary
  • Neoplasm Proteins
  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinase-3
  • Metalloendopeptidases

Associated data

  • GENBANK/X77690
  • GENBANK/X80791