A Herbicide Antidote (Safener) Induces the Activity of Both the Herbicide Detoxifying Enzyme and of a Vacuolar Transporter for the Detoxified Herbicide

FEBS Lett. 1994 Sep 26;352(2):219-21. doi: 10.1016/0014-5793(94)00961-9.

Abstract

In plants potentially toxic compounds are ultimately deposited in the large central vacuole. In this report we show that isolated barley mesophyll vacuoles take up the glucoside conjugate of the herbicide derivate [5-hydroxyphenyl]primisulfuron. Transport is stimulated by Mg-ATP and is distinct from that previously described for glutathione conjugates. Treatment of barley with different herbicide antidotes (safeners) revealed that the safener cloquintocet-mexyl doubles the vacuolar transport activities for both the glutathione and glucoside conjugates. Stimulation of the uptake of the metolachlor-glutathione conjugate was the result of an increased uptake velocity whereas the Km remained unaltered, suggesting that the higher activity was due to a higher expression of the transporter. These results indicate that modulation of vacuolar transport activities are an integral part of the detoxification mechanism of plants.

MeSH terms

  • Acetamides / metabolism
  • Biological Transport / drug effects
  • Biological Transport / physiology
  • Esculin / pharmacology
  • Glucose / analogs & derivatives
  • Glucose / metabolism
  • Glutathione / analogs & derivatives
  • Glutathione / metabolism
  • Glutathione Transferase / metabolism
  • Herbicides / metabolism*
  • Herbicides / pharmacology
  • Hordeum / metabolism*
  • Inactivation, Metabolic
  • Kinetics
  • Naphthalenes / pharmacology
  • Quinolines
  • Salicylates / pharmacology
  • Salicylic Acid
  • Vacuoles / metabolism*
  • Xenobiotics / metabolism

Substances

  • Acetamides
  • Herbicides
  • Naphthalenes
  • Quinolines
  • Salicylates
  • Xenobiotics
  • Esculin
  • 1,8-naphthalenedicarboxylic acid anhydride
  • cloquintocet-mexyl
  • Glutathione Transferase
  • Glutathione
  • Glucose
  • Salicylic Acid
  • metolachlor