Interleukin-8 Processing by Neutrophil Elastase, Cathepsin G and proteinase-3

FEBS Lett. 1994 Sep 26;352(2):231-5. doi: 10.1016/0014-5793(94)00952-x.

Abstract

Activated neutrophils secrete two forms of IL-8 with 77 and 72 amino acids, IL-8(77) and IL-8(72), along with proteinases that could process these cytokines. Significant conversion of IL-8(77) to more potent, N-terminally truncated forms was observed upon incubation with neutrophil granule lysates and purified proteinase-3. IL-8(72) was considerably more resistant to proteolytic processing than IL-8(77). The present observations indicate that neutrophil proteinases released in inflamed tissues convert IL-8 to more active forms and therefore tend to conserve or enhance, rather than decrease IL-8 activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cathepsin G
  • Cathepsins / metabolism*
  • Chemokine CXCL1
  • Chemokines, CXC*
  • Chemotactic Factors / metabolism
  • Chymotrypsin / metabolism
  • Extracellular Matrix Proteins / metabolism
  • Growth Substances / metabolism
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-8 / analogs & derivatives
  • Interleukin-8 / chemical synthesis
  • Interleukin-8 / genetics
  • Interleukin-8 / metabolism*
  • Leukocyte Elastase
  • Molecular Sequence Data
  • Myeloblastin
  • Neutrophil Activation*
  • Neutrophils / metabolism*
  • Pancreatic Elastase / metabolism*
  • Peptides / metabolism
  • Protein Structure, Tertiary
  • Serine Endopeptidases / metabolism*
  • Trypsin / metabolism
  • beta-Thromboglobulin

Substances

  • CXCL1 protein, human
  • Chemokine CXCL1
  • Chemokines, CXC
  • Chemotactic Factors
  • Extracellular Matrix Proteins
  • Growth Substances
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8
  • PPBP protein, human
  • Peptides
  • beta-Thromboglobulin
  • connective tissue-activating peptide
  • Cathepsins
  • Serine Endopeptidases
  • Chymotrypsin
  • CTSG protein, human
  • Cathepsin G
  • Pancreatic Elastase
  • Leukocyte Elastase
  • Trypsin
  • Myeloblastin