Monocyte chemotactic proteins MCP-1, MCP-2, and MCP-3 are major attractants for human CD4+ and CD8+ T lymphocytes

FASEB J. 1994 Oct;8(13):1055-60. doi: 10.1096/fasebj.8.13.7926371.


The responses of lymphocytes to six CC chemokines--MCP-1, MCP-2, MCP-3, MIP-1 alpha, MIP-1 beta, and RANTES--were studied using cloned human CD4+ and CD8+ T cells. All CC chemokines tested induced migration of both types of lymphocytes, whereas two CXC chemokines used as controls, IL-8 and IP-10, were inactive. The monocyte chemotactic proteins (MCP-1, MCP-2, and MCP-3) showed a typically bimodal concentration dependence, and were considerably more effective than MIP-1 alpha, MIP-1 beta, or RANTES. All CC chemokines also induced a rapid and transient rise in cytosolic free Ca2+ in either type of T cell. The rise was prevented by Bordetella pertussis toxin treatment, indicating that G-protein-coupled receptors are involved in signaling. It was most pronounced with MCP-1 and MCP-3, which is in agreement with the efficacy of these chemokines as chemoattractants. The responses to MCP-2, MIP-1 alpha, MIP-1 beta, and RANTES were weaker, and no changes were obtained on stimulation with IL-8 or IP-10. Freshly isolated human blood lymphocytes were also tested, but neither migration nor Ca2+ changes were observed. Low numbers of high-affinity receptors for MCP-1 were found on CD4+ and CD8+ cells ( < 900 per cell, Kd < 1 nM), and desensitization experiments showed that MCP-1, MCP-2, and MCP-3 share receptors. Owing to their superior effectiveness on CD4+ and CD8+ T cells, the monocyte chemotactic proteins could play a major role in the recruitment of activated T lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD4-Positive T-Lymphocytes / physiology
  • CD8-Positive T-Lymphocytes / physiology
  • Calcium / metabolism
  • Cells, Cultured
  • Chemokine CCL2
  • Chemokine CCL7
  • Chemokine CCL8
  • Chemotactic Factors / physiology*
  • Chemotaxis, Leukocyte / physiology*
  • Clone Cells
  • Cytokines*
  • Humans
  • Monocyte Chemoattractant Proteins*
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / physiology*
  • Time Factors


  • CCL7 protein, human
  • CCL8 protein, human
  • Chemokine CCL2
  • Chemokine CCL7
  • Chemokine CCL8
  • Chemotactic Factors
  • Cytokines
  • Monocyte Chemoattractant Proteins
  • Calcium