Localization of cholecystokinin A and cholecystokinin B/gastrin receptors in the canine upper gastrointestinal tract

Gastroenterology. 1994 Oct;107(4):1019-30. doi: 10.1016/0016-5085(94)90226-7.


Background/aims: Cholecystokinin (CCK) is a neuropeptide that exerts numerous effects in the gut. To determine the sites of action of CCK, the distribution and properties of CCK receptor subtypes were studied.

Methods: CCK receptors were localized by autoradiographic analysis of 125I-CCK binding to frozen sections of the canine upper gastrointestinal tract.

Results: In the cardiac and fundic stomach, CCK-B/gastrin receptors were found in the mucosa and in a subpopulation of neuronal elements in the circular muscle. The antrum expressed CCK-B/gastrin receptors in a few neurons in the circular muscle and in the entire myenteric plexus; no receptors were observed in the antral mucosa or esophagus. The duodenum showed a high concentration of CCK-B/gastrin receptors in the myenteric plexus. The cardiac and fundic basal mucosae expressed CCK-A receptors. Two nonpeptide CCK receptor antagonists were unable to differentiate between the receptor subtypes.

Conclusions: The differential expression of CCK receptor subtypes in the gastric mucosa provides a morphological basis for the separate regulatory roles of CCK and gastrin in gastric function. CCK-B/gastrin receptor expression in a subset of neurons in gastric circular muscle suggests a novel site of action for CCK and/or gastrin.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Autoradiography
  • Binding Sites
  • Cholecystokinin / metabolism
  • Densitometry
  • Digestive System / innervation
  • Digestive System / metabolism*
  • Dogs
  • Gastric Mucosa / metabolism
  • Myenteric Plexus / cytology
  • Myenteric Plexus / metabolism
  • Neurons / metabolism
  • Radioligand Assay
  • Receptors, Cholecystokinin / classification
  • Receptors, Cholecystokinin / metabolism*
  • Tissue Distribution


  • Receptors, Cholecystokinin
  • Cholecystokinin