pXeX, a vector for efficient expression of cloned sequences in Xenopus embryos

Gene. 1994 Sep 30;147(2):223-6. doi: 10.1016/0378-1119(94)90070-1.

Abstract

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1 alpha-encoding gene (EF-1 alpha). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Blastocyst
  • Cloning, Molecular
  • Culture Techniques
  • Embryo, Nonmammalian
  • Genetic Vectors*
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics
  • Plasmids*
  • RNA, Messenger / metabolism
  • Transcription, Genetic
  • Xenopus laevis

Substances

  • Peptide Elongation Factor 1
  • Peptide Elongation Factors
  • RNA, Messenger