Cloning and sequencing of cDNAs encoding the human hepatocyte nuclear factor 4 indicate the presence of two isoforms in human liver

Gene. 1994 Sep 30;147(2):269-72. doi: 10.1016/0378-1119(94)90079-5.


Hepatocyte nuclear factor 4 (HNF-4) is a key transcription factor involved in the specific expression of many genes in liver and intestine. Sequences of cDNAs coding for HNF-4 have been established in rat and Drosophila melanogaster. Rat HNF-4 exhibits two isoforms which probably result from differential splicing. We have isolated HNF-4 cDNAs from an adult human cDNA library. Sequence analysis revealed that two HNF-4 isoforms are also present in human liver. The complete sequence of the longest human isoform has been established and compared to the rat HNF-4 amino-acid sequences.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • Cloning, Molecular
  • DNA, Complementary
  • DNA-Binding Proteins*
  • Drosophila melanogaster / genetics
  • Hepatocyte Nuclear Factor 4
  • Humans
  • Liver / metabolism*
  • Molecular Sequence Data
  • Phosphoproteins*
  • Rats
  • Sequence Homology, Amino Acid
  • Transcription Factors / genetics*
  • Tumor Cells, Cultured


  • Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
  • DNA, Complementary
  • DNA-Binding Proteins
  • Hepatocyte Nuclear Factor 4
  • MLX protein, human
  • Phosphoproteins
  • Transcription Factors

Associated data

  • GENBANK/X76930