Construction of Improved Escherichia-Pseudomonas Shuttle Vectors Derived From pUC18/19 and Sequence of the Region Required for Their Replication in Pseudomonas Aeruginosa

Gene. 1994 Oct 11;148(1):81-6. doi: 10.1016/0378-1119(94)90237-2.

Abstract

The nucleotide sequence of the 1.9-kb PstI fragment from pRO1614, that allows stable maintenance of pMB1 (ColE1)-based cloning vectors in Pseudomonas, was determined. This fragment encodes a putative origin of replication (ori), a replication-controlling protein, and the C terminus of the Tn3 beta-lactamase-encoding gene. Improved versions of the broad-host-range plasmid vectors, pUCP18 and pUCP19, were constructed by deletion of nonessential DNA or replacement of nonessential DNA with an antibiotic-resistance cassette.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Replication / genetics*
  • Escherichia coli / genetics*
  • Genetic Vectors*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Plasmids / genetics
  • Pseudomonas aeruginosa / genetics*
  • Replication Origin / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • beta-Lactamases / genetics

Substances

  • beta-Lactamases

Associated data

  • GENBANK/L30112
  • GENBANK/U07164
  • GENBANK/U07165
  • GENBANK/U07166
  • GENBANK/U07167
  • GENBANK/U07168