Systemic release of interleukin-10 during orthotopic liver transplantation

Hepatology. 1994 Oct;20(4 Pt 1):889-92. doi: 10.1002/hep.1840200417.


Experimental and clinical observations indicate that the liver allograft is less immunogenic than other organ transplants and can promote immune tolerance. Because interleukin-10 recently emerged as a macrophage and T-cell-derived cytokine with potent immunosuppressive properties, we studied its production in 28 patients undergoing orthotopic liver transplantation. Plasma levels of immunoreactive interleukin-10 dramatically increased within 2 hr after liver allograft reperfusion, with peak levels ranging between 214 and 4998 pg/ml (median = 677 pg/ml). This systemic release of interleukin-10 was transient because it returned to low levels by 48 hr (range = 26 to 51 pg/ml). The higher interleukin-10 levels measured in right atrial blood as compared with portal blood indicated that interleukin-10 was most likely synthesized within the liver graft. To get insight into the cellular origin of interleukin-10, we also measured serum levels of interleukin-4 and interferon-gamma, both produced by T cells, and interleukin-8, a cytokine secreted by macrophages, in eight patients. Interleukin-4 and interferon-gamma levels remained undetectable in most of the patients, whereas interleukin-8 levels paralleled those of interleukin-10. Portal endotoxemia was probably not involved in interleukin-10 production because endotoxin levels remained low (< 20 pg/ml) before and after liver allograft reperfusion. Interleukin-10 plasma levels did not correlate either with cold ischemia time or with the occurrence of rejection episodes. We conclude that orthotopic liver transplantation is associated with a massive release of interleukin-10 and interleukin-8, most likely produced by allograft macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Endotoxins / blood
  • Female
  • Humans
  • Interferon-gamma / biosynthesis
  • Interleukin-10 / biosynthesis*
  • Interleukin-4 / biosynthesis
  • Interleukin-8 / biosynthesis
  • Liver / immunology
  • Liver / metabolism
  • Liver Transplantation / immunology*
  • Macrophages / metabolism
  • Male
  • Middle Aged
  • Regression Analysis
  • T-Lymphocytes / metabolism


  • Endotoxins
  • Interleukin-8
  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma