Excitatory amino acid receptors in glial progenitor cells: molecular and functional properties

Glia. 1994 Jun;11(2):94-101. doi: 10.1002/glia.440110204.


We have analyzed the molecular and biophysical properties of glutamate-gated channels in cells of the oligodendrocyte lineage, using both the CG-4 primary cell line (Louis et al: J. Neurosci. Res. 31:193-204, 1992a) and oligodendrocyte progenitors purified from the rat cerebral cortex. CG-4 progenitor cells, as well as primary progenitors, were stained with a specific anti-GABA antibody. In whole-cell patch-clamp recordings, rapid perfusion of the agonists L-glutamate, kainate, and AMPA produced rapidly desensitizing currents in CG-4 cells. NMDA was ineffective. Both rapidly desensitizing and steady-state components of responses to kainate were inhibited by the kainate/AMPA receptor antagonist CNQX. Northern blot analysis of total mRNA isolated from CG-4 cells revealed co-expression of both AMPA- and kainate-preferring glutamate receptor subunits. The activation of glutamate receptors in CG-4 cells caused a rapid and transient elevation of mRNAs for the immediate early gene NGFI-A.

MeSH terms

  • 6-Cyano-7-nitroquinoxaline-2,3-dione / pharmacology
  • Animals
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • DNA-Binding Proteins / biosynthesis
  • Early Growth Response Protein 1
  • Gene Expression Regulation / drug effects
  • Genes, Immediate-Early / drug effects
  • Glutamic Acid / pharmacology*
  • Immediate-Early Proteins*
  • Kainic Acid / pharmacology
  • Membrane Potentials / drug effects
  • N-Methylaspartate / pharmacology
  • Nerve Tissue Proteins / physiology*
  • Neurotoxins / pharmacology*
  • Oligodendroglia / drug effects
  • Oligodendroglia / physiology*
  • Rats
  • Receptors, Glutamate / drug effects
  • Receptors, Glutamate / physiology*
  • Stem Cells / drug effects
  • Stem Cells / physiology*
  • Transcription Factors / biosynthesis


  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, rat
  • Immediate-Early Proteins
  • Nerve Tissue Proteins
  • Neurotoxins
  • Receptors, Glutamate
  • Transcription Factors
  • Glutamic Acid
  • N-Methylaspartate
  • 6-Cyano-7-nitroquinoxaline-2,3-dione
  • Kainic Acid