Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE binding to group I allergens from eight different grass species

J Allergy Clin Immunol. 1994 Oct;94(4):689-98. doi: 10.1016/0091-6749(94)90176-7.


Background: Grass pollens, such as pollen from timothy grass (Phleum pratense), represent a major cause of type I allergy.

Objective: In this report we attempted to determine how cross-reactive allergenic components of grass pollens from different species can be represented by a minimum number of recombinant allergens.

Methods: We isolated and sequenced a timothy grass pollen cDNA coding for the major allergen Phl p I. A recombinant Phl p I-beta-galactosidase fusion protein, which bound to IgE in 87% of patients with grass pollen allergy, was produced in Escherichia coli. Using recombinant Phl p V and Phl p I, we defined representative patients' sera that bound to group I but not to group V allergens, as well as sera with reactivity against group I and group V allergens. IgE immunoblot inhibition studies were done with nitrocellulose-blotted pollen extracts from eight grass species with different geographic distribution.

Results: Preadsorption of patients' sera with recombinant nonfusion Phl p I strongly reduced IgE binding to group I allergens from the eight grasses, showing extensive cross-reactivity between species.

Conclusion: A single recombinant group I allergen contains many of the IgE epitopes of group I isoallergens from a number of different grass species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics*
  • Allergens / pharmacology*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • DNA, Complementary / genetics*
  • Humans
  • Immunoblotting
  • Immunoglobulin E / immunology*
  • Molecular Sequence Data
  • Plant Extracts / pharmacology
  • Poaceae / immunology*
  • Pollen / immunology*
  • Recombinant Proteins


  • Allergens
  • DNA, Complementary
  • Plant Extracts
  • Recombinant Proteins
  • Immunoglobulin E

Associated data

  • GENBANK/X78813