Aberrations of the tumor suppressor p53 gene and p53 protein in solar keratosis in human skin

J Invest Dermatol. 1994 Oct;103(4):500-3. doi: 10.1111/1523-1747.ep12395643.


Aberrations of the p53 gene in 27 solar keratoses were examined by the polymerase chain reaction and single-strand conformation polymorphism and DNA sequencing analyses. In a series of Japanese patients, eight of 27 cases (30%) of solar keratosis showed structural abnormalities of the p53 gene. Six of eight aberrations of p53 gene were determined to be single nucleotide substitutions, and five of these were located at a dipyrimidine site. In solar keratosis, noticeable mutations were C to T in three cases, and one each of C to A and T to C nucleotide changes. p53 protein was detected immunohistochemically in the nuclei of six of 27 cases (22%) of solar keratosis. Nuclear staining for p53 protein was only significantly correlated with the presence of missense mutation of p53 gene (p < 0.01). Aberrations of the p53 gene in solar keratosis may be a marker to predict early cancerous lesions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosome Aberrations*
  • Genes, p53 / genetics*
  • Humans
  • Immunohistochemistry
  • Keratosis / etiology
  • Keratosis / genetics*
  • Molecular Sequence Data
  • Mutation
  • Polymorphism, Single-Stranded Conformational
  • Tumor Suppressor Protein p53 / analysis
  • Ultraviolet Rays / adverse effects*


  • Tumor Suppressor Protein p53