Rapid accumulation of fluorescent material with aging in an oxygen-sensitive mutant mev-1 of Caenorhabditis elegans

Mech Ageing Dev. 1994 Jun;74(3):161-70. doi: 10.1016/0047-6374(94)90087-6.

Abstract

Mutations in mev-1 of the nematode Caenorhabditis elegans render animals hypersensitive to high oxygen concentrations. They also reduce life span. To further understand the effects of mev-1 on aging, accumulation of fluorescent material resembling lipofuscin was measured by biochemical and histological analyses. Fluorescent material accumulated in both wild type and mev-1 animals with increasing age. The mev-1 mutant accumulated more fluorescent material at a greater rate than dose wild type. Furthermore, the accumulation rates depended on concentration of oxygen. Since this phenotype has been widely used as an aging marker, these results validate mev-1's use as a model to study aging.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / metabolism*
  • Animals
  • Caenorhabditis elegans / drug effects
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism*
  • Drug Resistance
  • Fluorescence
  • Fluorometry
  • Mutation*
  • Oxygen / pharmacology*
  • Reference Values

Substances

  • Oxygen