Secondary-sites for integration mediated by the Tn21 integrase

Mol Microbiol. 1993 Nov;10(4):823-8. doi: 10.1111/j.1365-2958.1993.tb00952.x.


The integrase encoded by the integron of the transposon Tn21 can mediate the site-specific fusion of two plasmids if there is a recombination hot spot (59 bp element) in one of them and the sequence GWTMW in the other. The use of this latter, loosely defined site explains how antibiotic-resistance genes could first become associated with integrons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites / genetics
  • DNA Nucleotidyltransferases / genetics*
  • DNA Nucleotidyltransferases / metabolism
  • DNA Transposable Elements*
  • DNA, Bacterial / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics*
  • Integrases
  • Molecular Sequence Data
  • Plasmids / genetics
  • Recombination, Genetic


  • DNA Transposable Elements
  • DNA, Bacterial
  • DNA Nucleotidyltransferases
  • Integrases