Targeted mutagenesis of the phospholipase D gene results in decreased virulence of Corynebacterium pseudotuberculosis

Mol Microbiol. 1994 Jun;12(6):921-30. doi: 10.1111/j.1365-2958.1994.tb01080.x.


The chromosomal gene encoding the phospholipase D from Corynebacterium pseudotuberculosis (biovar ovis) isolate Whetten 1 was replaced with an allele containing a nonsense mutation. The virulence of the mutant strain (W1.31r1) and the isogenic parental strain were then compared by inoculation of goats. The wild-type strain caused abscessation at the site of infection, which then spread to the regional lymph node, while W1.31r1 had a reduced ability to establish a primary infection and was incapable of dissemination. Our results confirm that phospholipase D is a virulence determinant of C. pseudotuberculosis that increases the persistence and spread of the bacteria within the host.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Corynebacterium Infections / microbiology
  • Corynebacterium Infections / veterinary
  • Corynebacterium pseudotuberculosis / enzymology
  • Corynebacterium pseudotuberculosis / genetics
  • Corynebacterium pseudotuberculosis / growth & development
  • Corynebacterium pseudotuberculosis / pathogenicity*
  • Gene Targeting
  • Goat Diseases / microbiology
  • Goats
  • Lymph Nodes / microbiology
  • Male
  • Molecular Sequence Data
  • Mutagenesis*
  • Mutation / physiology*
  • Phospholipase D / genetics*
  • Virulence


  • Phospholipase D

Associated data

  • GENBANK/L16587