Measurement of receptor-stimulated guanosine 5'-O-(gamma-thio)triphosphate binding by G proteins

Methods Enzymol. 1994;237:3-13. doi: 10.1016/s0076-6879(94)37048-6.
No abstract available

MeSH terms

  • Animals
  • Binding, Competitive
  • Cell Membrane / metabolism
  • Centrifugation, Density Gradient / methods
  • Erythrocyte Membrane / metabolism
  • GTP-Binding Proteins / isolation & purification
  • GTP-Binding Proteins / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism*
  • Guanosine Diphosphate / pharmacology
  • Humans
  • Indicators and Reagents
  • Kinetics
  • Macromolecular Substances
  • Radioisotope Dilution Technique
  • Receptors, Adrenergic, beta / metabolism
  • Receptors, Cell Surface / agonists
  • Receptors, Cell Surface / metabolism*
  • Receptors, Muscarinic / metabolism
  • Sodium Chloride / pharmacology
  • Sulfur Radioisotopes
  • Temperature
  • Turkeys

Substances

  • Indicators and Reagents
  • Macromolecular Substances
  • Receptors, Adrenergic, beta
  • Receptors, Cell Surface
  • Receptors, Muscarinic
  • Sulfur Radioisotopes
  • Guanosine Diphosphate
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Sodium Chloride
  • GTP-Binding Proteins