Single-stranded DNA-protein binding in the procyclic acidic repetitive protein (PARP) promoter of Trypanosoma brucei

Mol Biochem Parasitol. 1994 May;65(1):109-22. doi: 10.1016/0166-6851(94)90120-1.


We performed gel retardation analyses of DNA-protein interactions using DNA from the procyclic acidic repetitive protein (PARP) promoter of the protozoan parasite Trypanosoma brucei. The PARP genes of Trypanosoma brucei are transcribed in an alpha-amanitin resistant manner, and it has been proposed that RNA polymerase I, rather than RNA polymerase II, transcribes the PARP genes. Double-stranded restriction fragments containing the essential PARP-promoter regions bound only sequence-nonspecific nuclear factors, even though protein factors that bind specifically to double-stranded DNA from the snRNA U2 promoter were present in the extracts. In contrast, single-stranded DNA-binding proteins bound with high affinity, nucleotide-sequence and strand-specificity to the -69/-55 element and the coding and non-coding strands of the -37/-11 element.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • DNA Probes / genetics
  • DNA, Protozoan / genetics*
  • DNA, Protozoan / metabolism*
  • DNA, Single-Stranded / genetics
  • DNA, Single-Stranded / metabolism
  • DNA-Binding Proteins / metabolism
  • Genes, Protozoan
  • Membrane Glycoproteins*
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Protein Binding
  • Protozoan Proteins / metabolism
  • Trypanosoma brucei brucei / genetics*
  • Trypanosoma brucei brucei / metabolism*
  • Variant Surface Glycoproteins, Trypanosoma / genetics*


  • DNA Probes
  • DNA, Protozoan
  • DNA, Single-Stranded
  • DNA-Binding Proteins
  • Membrane Glycoproteins
  • Protozoan Proteins
  • Variant Surface Glycoproteins, Trypanosoma
  • procyclic acidic repetitive protein, Trypanosoma