Activation of Raf-1 by 14-3-3 proteins

Nature. 1994 Oct 13;371(6498):612-4. doi: 10.1038/371612a0.

Abstract

The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 14-3-3 Proteins
  • Animals
  • CDC2 Protein Kinase / metabolism
  • Cells, Cultured
  • Enzyme Activation
  • Nerve Tissue Proteins / metabolism*
  • Oocytes
  • Oogenesis / genetics
  • Oogenesis / physiology
  • Protein-Serine-Threonine Kinases / metabolism*
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-raf
  • Recombinant Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Tyrosine 3-Monooxygenase*
  • Xenopus

Substances

  • 14-3-3 Proteins
  • Nerve Tissue Proteins
  • Proto-Oncogene Proteins
  • Recombinant Proteins
  • Tyrosine 3-Monooxygenase
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-raf
  • CDC2 Protein Kinase