Changes in membrane proteins of Escherichia coli K12 mediated by bacteriophage IKe-specific plasmids

Biochim Biophys Acta. 1976 Dec 22;453(2):311-8. doi: 10.1016/0005-2795(76)90126-4.

Abstract

At least 3 minor proteins have been detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis in the unseparated (31 000 daltons) and inner membranes (53 000 and 86 000 daltons) of a non-piliated strain of E. coli K12 bearing the phage IKe-specific resistance plasmid RM98 (Tra+); these proteins are lacking in its Tra- derivative and in the plasmidless host. In contrast, in another K12 host carrying each of different IKe-specific colicinogenic factors, an extra 30 000 dalton protein is observed in the outer membranes; a number of minor proteins seen in the inner membranes appear to be different from those observed in the RM98+ strain. The evidence suggests that the 30 000-31 000 dalton protein is transfer function specific.

MeSH terms

  • Coliphages / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / metabolism*
  • Extrachromosomal Inheritance*
  • Membrane Proteins / metabolism*
  • Molecular Weight
  • Mutation
  • Plasmids*
  • Species Specificity
  • Virus Replication

Substances

  • Membrane Proteins