Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases

Nucleic Acids Res. 1994 Sep;22(17):3640-59. doi: 10.1093/nar/22.17.3640.


Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known site-specific DNA modification methyltransferases and their specificities is presented along with EMBL database accession numbers for cloned genes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacteria / enzymology
  • Base Sequence
  • DNA / metabolism
  • DNA Modification Methylases / genetics*
  • DNA Modification Methylases / metabolism
  • DNA Restriction Enzymes / genetics*
  • DNA Restriction Enzymes / metabolism
  • Hydrolysis
  • Kinetics
  • Methylation
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides


  • Oligodeoxyribonucleotides
  • DNA
  • DNA Modification Methylases
  • DNA Restriction Enzymes