[The polymorphism typing of the HLA-DQ-alpha gene by enzymatic amplification in studying material evidence of biological origin]

I V Ovchinnikov; Iu I Savel'ev; A V Kalashnikov; M V Chelnokova; V V Nosikov; V G Debabov

[The polymorphism typing of the HLA-DQ-alpha gene by enzymatic amplification in studying material evidence of biological origin]

Sud Med Ekspert. 1993 Apr-Jun;36(2):32-5.

[Article in Russian]

Authors

I V Ovchinnikov, Iu I Savel'ev, A V Kalashnikov, M V Chelnokova, V V Nosikov, V G Debabov

PMID: 7940632

Abstract

The method of DNA enzymatic amplification on the basis of polymerase chain reaction is described and genetic marker system criteria are defined. The method is highly sensitive. It permits gene polymorphism typing if only solitary cells are available and in case DNA is degraded. Practical use of the method is described.

Publication types

English Abstract

MeSH terms

Alleles
Base Sequence
Blood Stains
DNA / genetics
DNA / isolation & purification
Forensic Medicine / methods*
Genotype
HLA-DQ Antigens / genetics*
HLA-DQ alpha-Chains
Hair
Humans
Male
Molecular Sequence Data
Nucleic Acid Amplification Techniques*
Oligonucleotide Probes
Polymorphism, Genetic*
Spermatozoa

Substances

HLA-DQ Antigens
HLA-DQ alpha-Chains
HLA-DQA1 antigen
Oligonucleotide Probes
DNA