The two-hybrid system: an assay for protein-protein interactions

Trends Genet. 1994 Aug;10(8):286-92. doi: 10.1016/0168-9525(90)90012-u.


The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions. It can be used to identify proteins that bind to a protein of interest, or to delineate domains or residues critical for an interaction. Variations on this methodology have been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA-Binding Proteins / metabolism*
  • Protein Binding
  • Proteins / metabolism*


  • DNA-Binding Proteins
  • Proteins