Abstract
Differential screening procedure was employed to isolate new genes specifically down-regulated in RSV-transformed rat 3Y1 cells (SR-3Y1) compared with parental 3Y1 cells. Among those, one clone termed N03 showed nearly complete down-regulation in v-src-, v-mos and SV40-transformed 3Y1 cells. Nucleotide sequence analysis showed that N03 protein is novel and is composed of 178 amino acid residues in length. Introduction of N03 cDNA into SR-3Y1 cells using an expression vector resulted in suppression of transformed phenotypes such as high growth rate, colony-forming ability in soft agar and tumorigenicity in nude mice.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Cycle Proteins
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Cell Transformation, Neoplastic / genetics*
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Cells, Cultured
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Cloning, Molecular
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DNA, Complementary / genetics
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Down-Regulation
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Gene Expression Regulation, Neoplastic / genetics*
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Genes, Tumor Suppressor / genetics*
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Genes, src / genetics
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Humans
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Lung / chemistry
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Lung Neoplasms / chemistry
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Molecular Sequence Data
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Nerve Tissue Proteins
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Proteins / genetics*
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Rats
Substances
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Cell Cycle Proteins
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DNA, Complementary
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NBL1 protein, human
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Nbl1 protein, rat
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Nerve Tissue Proteins
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Proteins
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RNA, Messenger