Molecular cloning and characterization of a cDNA showing tumor-suppressive activity in V-SRC-transformed 3Y1 rat fibroblasts

Adv Enzyme Regul. 1994:34:247-55. doi: 10.1016/0065-2571(94)90019-1.

Abstract

Differential screening procedure was employed to isolate new genes specifically down-regulated in RSV-transformed rat 3Y1 cells (SR-3Y1) compared with parental 3Y1 cells. Among those, one clone termed N03 showed nearly complete down-regulation in v-src-, v-mos and SV40-transformed 3Y1 cells. Nucleotide sequence analysis showed that N03 protein is novel and is composed of 178 amino acid residues in length. Introduction of N03 cDNA into SR-3Y1 cells using an expression vector resulted in suppression of transformed phenotypes such as high growth rate, colony-forming ability in soft agar and tumorigenicity in nude mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle Proteins
  • Cell Transformation, Neoplastic / genetics*
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic / genetics*
  • Genes, Tumor Suppressor / genetics*
  • Genes, src / genetics
  • Humans
  • Lung / chemistry
  • Lung Neoplasms / chemistry
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Proteins / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Rats

Substances

  • Cell Cycle Proteins
  • DNA, Complementary
  • NBL1 protein, human
  • Nbl1 protein, rat
  • Nerve Tissue Proteins
  • Proteins
  • RNA, Messenger