Metabolic effects and fate of succinic acid methyl esters in rat hepatocytes

Arch Biochem Biophys. 1994 Oct;314(1):186-92. doi: 10.1006/abbi.1994.1428.


The metabolic fate and metabolic effects of succinic acid methyl esters were examined in rat isolated hepatocytes. Both the monomethyl ester (SAM) and dimethyl ester (SAD) of succinic acid inhibited D-glucose metabolism. Such an inhibition affected, in order of increasing severity, the direct incorporation of D-glucose into glycogen and futile cycling between the hexose and its 6-phosphate ester, the phosphorylation of D-glucose, the generation of triose phosphates from the hexose, and the production of 14C-labeled lactate, pyruvate, and amino acids from D-[U-14C]glucose and its oxidation. The dimethyl ester of [1,4-14C]succinic acid ([1,4-14C]SAD) or [2,3-14C]-succinic acid ([2,3-14C]SAD) was efficiently converted to acidic metabolites. The oxidation of [1,4-14C]SAD largely exceeded that of [2,3-14C]SAD. Inversely the generation of newly formed radioactive D-glucose and glycogen appeared higher in cells exposed to [2,3-14C]SAD, rather than [1,4-14C]SAD. It is proposed that SAM and SAD are suitable nutrients both to cover the energy need of hepatocytes and to act as gluconeogenic precursors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / metabolism
  • Animals
  • Esters / metabolism
  • Female
  • Gluconeogenesis
  • Glucose / metabolism
  • Glycogen / metabolism
  • Lactates / metabolism
  • Lactic Acid
  • Liver / drug effects
  • Liver / metabolism*
  • Oxidation-Reduction
  • Phosphorylation
  • Pyruvates / metabolism
  • Pyruvic Acid
  • Rats
  • Succinates / metabolism
  • Succinates / pharmacology*


  • Amino Acids
  • Esters
  • Lactates
  • Pyruvates
  • Succinates
  • Lactic Acid
  • Pyruvic Acid
  • Glycogen
  • dimethyl succinate
  • Glucose
  • monomethyl succinate