Lung preservation with Euro-Collins, University of Wisconsin, Wallwork, and low-potassium-dextran solution. Université++ Paris-Sud Lung Transplant Group

Ann Thorac Surg. 1994 Sep;58(3):845-50. doi: 10.1016/0003-4975(94)90765-x.

Abstract

Using isolated rat lungs, we compared prevention of ischemia-reperfusion injury provided by flushing the lungs with modified Euro-Collins solution (EC), University of Wisconsin solution (UW), low-potassium-dextran solution (LPD), or Wallwork solution (WA). After 4 hours' and 6 hours' cold ischemia, reperfusion injury was assessed on the basis of changes in filtration coefficients (Kfc) and pressure-flow curves, characterized by the slope of the curves (incremental resistance) and the extrapolation of this slope to zero flow (pulmonary pressure intercept [Ppi]). After 4 hours, Kfc and Ppi were higher with EC than with UW, LPD, and WA, and the incremental resistance was higher with EC and UW. After 6 hours, Kfc and incremental resistance Ppi were higher with LPD than with WA. Because ischemia-reperfusion injury is associated with decreased endothelial synthesis of prostacyclin and nitric oxide, we tested whether the addition of prostacyclin or the nitric oxide precursor L-arginine to WA would improve preservation. The Kfc and Ppi were lower with both treatments. In conclusion, ischemia-reperfusion injury was best prevented by using WA. The favorable effect of prostacyclin or L-arginine emphasizes the role played by endothelial dysfunction in ischemia-reperfusion injury.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine / administration & dosage
  • Albumins / administration & dosage*
  • Allopurinol / administration & dosage
  • Animals
  • Blood Pressure
  • Cardioplegic Solutions / administration & dosage
  • Chlorides / administration & dosage*
  • Epoprostenol / administration & dosage
  • Glutathione / administration & dosage
  • Hypertonic Solutions / administration & dosage*
  • Hypothermia, Induced
  • In Vitro Techniques
  • Insulin / administration & dosage
  • Lung / blood supply*
  • Lung / physiopathology
  • Lung Compliance
  • Male
  • Mannitol / administration & dosage*
  • Organ Preservation / methods*
  • Organ Preservation Solutions*
  • Organ Size
  • Potassium Compounds / administration & dosage*
  • Propionates / administration & dosage*
  • Pulmonary Artery / physiopathology
  • Raffinose / administration & dosage
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / prevention & control*
  • Time Factors
  • Vascular Resistance

Substances

  • Albumins
  • Cardioplegic Solutions
  • Chlorides
  • Euro-Collins' solution
  • Hypertonic Solutions
  • Insulin
  • Organ Preservation Solutions
  • Potassium Compounds
  • Propionates
  • University of Wisconsin-lactobionate solution
  • Wallwork solution
  • potassium cardioplegic solution
  • Mannitol
  • Allopurinol
  • Epoprostenol
  • Glutathione
  • Adenosine
  • Raffinose