Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C

Neuron. 1994 Oct;13(4):885-98. doi: 10.1016/0896-6273(94)90254-2.


rab3A, a low molecular weight GTP-binding protein of synaptic vesicles with a putative function in synaptic vesicle docking, interacts in a GTP-dependent manner with rabphilin-3A, a peripheral membrane protein that binds Ca2+ and phospholipids. We now show that rabphilin-3A is an evolutionarily conserved synaptic vesicle protein that is attached to synaptic vesicle membranes via its N terminus and exhibits a heterogeneous distribution among synapses. In rab3A-deficient mice, rabphilin-3A is decreased in synapses belonging to neurons that primarily express rab3A and accumulates in the perikarya of these neurons. In contrast, neurons expressing significant levels of rab3C still contain normal levels of rabphilin-3A in a synaptic pattern, and rabphilin-3A binds rab3C in vitro. These results suggest that analogous to the membrane recruitment of raf by ras, rab3A and rab3C may function in recruiting rabphilin-3A to the synaptic vesicle membrane in a GTP-dependent manner.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Brain Chemistry
  • Conserved Sequence
  • DNA, Complementary / chemistry
  • Fluorescent Antibody Technique
  • GTP-Binding Proteins / analysis
  • GTP-Binding Proteins / deficiency
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • GTP-Binding Proteins / physiology*
  • Glutathione Transferase / genetics
  • Mice
  • Mice, Mutant Strains
  • Microscopy, Immunoelectron
  • Molecular Sequence Data
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Nerve Tissue Proteins / physiology*
  • Neurons / chemistry
  • Rats
  • Recombinant Fusion Proteins
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins*
  • rab3 GTP-Binding Proteins


  • Adaptor Proteins, Signal Transducing
  • DNA, Complementary
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Vesicular Transport Proteins
  • rabphilin-3A
  • Glutathione Transferase
  • GTP-Binding Proteins
  • rab GTP-Binding Proteins
  • rab3 GTP-Binding Proteins

Associated data

  • GENBANK/U12571