Frequency of allele loss of DCC, p53, RBI, WT1, NF1, NM23 and APC/MCC in colorectal cancer assayed by fluorescent multiplex polymerase chain reaction

Br J Cancer. 1994 Nov;70(5):813-8. doi: 10.1038/bjc.1994.404.


We report here the use of multiplex fluorescent polymerase chain reaction (PCR) for quantitative allele loss detection using microsatellites with 2-5 base pair repeat motifs. Allele loss of APC, DCC, p53 and RB1 in colorectal tumours has been reported previously using a variety of methods. However, not all workers used intragenic markers. We have used microsatellite polymorphisms which map within, or are closely linked to, these tumour-suppressor gene loci in order to determine whether these loci are indeed the targets for alteration in colorectal cancer. In addition, we have assayed two other tumour-suppressor genes, WT1 and NF1, to see whether they play a role in colorectal carcinogenesis. The putative metastasis-suppressor gene, NM23, was also investigated since there have been conflicting reports about its involvement in colorectal carcinogenesis. Allele loss was detected at the DCC (29%), p53 (66%), RB1 (50%) and NF1 (14%) loci and in the APC/MCC region (50%), but not at the WT1 or NM23 loci. These rapid, and mostly gene-specific, fluorescent multiplex PCR assays for allele loss detection could be modified to devise a single molecular diagnostic test for the important lesions in colorectal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics*
  • Adult
  • Aged
  • Aged, 80 and over
  • Alleles*
  • Base Sequence
  • Colorectal Neoplasms / genetics*
  • DNA, Satellite / analysis
  • DNA, Satellite / genetics
  • Female
  • Fluorescence
  • Gene Deletion*
  • Genes, APC / genetics
  • Genes, DCC / genetics
  • Genes, MCC / genetics
  • Genes, Neurofibromatosis 1 / genetics
  • Genes, Retinoblastoma / genetics
  • Genes, Tumor Suppressor*
  • Genes, Wilms Tumor
  • Genes, p53 / genetics
  • Genetic Markers
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Monomeric GTP-Binding Proteins*
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside-Diphosphate Kinase*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic
  • Sensitivity and Specificity
  • Transcription Factors / genetics


  • DNA, Satellite
  • Genetic Markers
  • NM23 Nucleoside Diphosphate Kinases
  • Transcription Factors
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins