Galectin-3 is a novel substrate for human matrix metalloproteinases-2 and -9

Biochemistry. 1994 Nov 29;33(47):14109-14. doi: 10.1021/bi00251a020.

Abstract

The primary structure of galectin-3, a approximately 30 kDa galactoside-binding protein (aka CBP-35, mL-34, hL-31, L-29, Mac-2, and epsilon BP), reveals two structural domains: an amino-terminal domain consists of a Pro-Gly-rich motif, and a globular carboxyl-terminal domain containing a carbohydrate-binding site. In this study, we report that the amino-terminal domain of galectin-3 contains a cleavage site for two members of the matrix metalloproteinase family of enzymes: the 72 kDa (gelatinase A, MMP-2) and the 92 kDa (gelatinase B, MMP-9) proteinases. The major cleavage site for the gelatinases in galectin-3 is at the Ala62-Tyr63 bond, and its hydrolysis by these enzymes was inhibited by TIMP-2. Cell-surface expression of galectin-3 was reduced following treatment of viable T47D human breast carcinoma cells with gelatinase A. These results suggest that galectin-3 may be a substrate for gelatinases and that its degradation may play a role in modulating the biological activities of galectin-3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Differentiation / chemistry
  • Antigens, Differentiation / metabolism*
  • Binding Sites
  • Breast Neoplasms / metabolism
  • Carbohydrate Metabolism
  • Collagenases / metabolism*
  • Galectin 3
  • Gelatinases / antagonists & inhibitors
  • Gelatinases / metabolism*
  • Humans
  • Hydrolysis
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9
  • Matrix Metalloproteinase Inhibitors
  • Metalloendopeptidases / antagonists & inhibitors
  • Metalloendopeptidases / metabolism*
  • Molecular Sequence Data
  • Proteins / pharmacology
  • Substrate Specificity
  • Tissue Inhibitor of Metalloproteinase-2
  • Tumor Cells, Cultured

Substances

  • Antigens, Differentiation
  • Galectin 3
  • Matrix Metalloproteinase Inhibitors
  • Proteins
  • Tissue Inhibitor of Metalloproteinase-2
  • Collagenases
  • Gelatinases
  • Metalloendopeptidases
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9

Associated data

  • GENBANK/M64303