cDNA sequence for bovine ribosomal protein L3 carrying a bipartite nuclear targeting motif, identified also in many other ribosomal proteins

Biochim Biophys Acta. 1994 Nov 22;1219(3):706-10. doi: 10.1016/0167-4781(94)90232-1.


A full-length cDNA encoding bovine ribosomal protein L3 was isolated and sequenced. The deduced protein sequence comprises 403 amino acids and shows a high level of identity with the other known mammalian L3 proteins. Southern blot analysis of bovine genomic DNA suggests that the bovine genome contains at least 4 copies of the L3 gene. A single hybridisation band of about 1.3 kb is detectable by Northern blot analysis. Within the amino acid sequence, two potential nuclear targeting sequences were detected: one at the N-terminal end and the other, consisting in a bipartite motif (amino acids 341 to 358), present and not previously noticed also in the other known mammalian L3 proteins. A search on all the available mammalian ribosomal proteins revealed the presence of this bipartite motif in many of these proteins.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • Blotting, Northern
  • Cattle
  • Cell Compartmentation / genetics*
  • Cell Nucleus / metabolism*
  • DNA, Complementary / genetics
  • Molecular Sequence Data
  • Protein Sorting Signals / genetics
  • RNA, Messenger / genetics
  • Ribosomal Proteins / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Protein Sorting Signals
  • RNA, Messenger
  • Ribosomal Proteins
  • ribosomal protein L3

Associated data

  • GENBANK/Z29555