Resistance to degradation by nucleases of (2'S)-2'-deoxy-2'-C-methyloligonucleotides, novel potential antisense probes

Antisense Res Dev. Summer 1994;4(2):95-8. doi: 10.1089/ard.1994.4.95.


An oligonucleotide must fulfill two main requirements to become a potential antisense probe: effective hybridization properties with the complementary sequence and stability toward nucleases. In this article the degradation pattern of a new class of potential antisense fragment, (2'S)-2'-deoxy-2'-C-methyloligonucleotides, is analyzed. The results described here show that the modification introduced in these oligonucleotides confers an enhanced stability toward purified nucleases, human sera, and HeLa cell extracts.

MeSH terms

  • Base Sequence
  • Deoxyribonucleases / chemistry*
  • HeLa Cells
  • Humans
  • Methylation
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / chemistry*
  • Ribonucleases / chemistry*


  • Oligonucleotides, Antisense
  • Deoxyribonucleases
  • Ribonucleases