Isolation and characterization of group-I isoallergens from Bermuda grass pollen

Int Arch Allergy Immunol. 1994 May;104(1):57-64. doi: 10.1159/000236709.

Abstract

Two isoallergens of Cyn d I were isolated using preparative isoelectric focussing, and were designated Cyn d Ia and b. These isoallergens differ in their pI, molecular weight (Cyn d Ia, 32 kD and Cyn d Ib, 31 kD) and their NH2-terminal sequence. Four monoclonal antibodies (Mabs) were produced using Cyn d Ia as antigen. These Mabs recognized both Cyn d Ia and b. One of the Mabs recognized four different pI forms of Cyn d I on 2D gels. The Mabs also recognized cross-reactive epitopes on proteins from eight other grasses including rye grass, timothy grass, Kentucky bluegrass and Johnson grass.

MeSH terms

  • Allergens / chemistry
  • Allergens / immunology*
  • Allergens / isolation & purification*
  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, Plant
  • Blotting, Western
  • Cross Reactions
  • Electrophoresis, Gel, Two-Dimensional
  • Electrophoresis, Polyacrylamide Gel
  • Isoelectric Focusing
  • Molecular Sequence Data
  • Plant Proteins / chemistry
  • Plant Proteins / immunology*
  • Plant Proteins / isolation & purification*
  • Pollen / chemistry
  • Pollen / immunology*

Substances

  • Allergens
  • Antibodies, Monoclonal
  • Antigens, Plant
  • CYND1 protein, Cynodon dactylon
  • Plant Proteins