n-Butyrate enhances induction of thyroid hormone-responsive nuclear protein

Endocr J. 1993 Oct;40(5):515-21. doi: 10.1507/endocrj.40.515.


Effects of n-butyrate on nuclear thyroid hormone receptors and on thyroid hormone-responsive nuclear protein were investigated by means of a perfusion system in rat liver. Treatment with 5 mM n-butyrate resulted in an increase (150%) in the maximal binding capacity of 3,5,3'-L-triiodothyronine (T3) nuclear receptors without altering the affinity of receptor for T3. However, further perfusion for 4 h decreased the number of the receptors to the control level. n-Butyrate increased the amount of acetylated histone H4. The ability of nuclear T3 receptors to bind to core histones was diminished by acetylation of the core histones. Thyroid hormone-responsive nuclear protein (n protein) was increased by T3. The induction of the n protein by T3 was augmented by n-butyrate. These results suggested that n-butyrate modulates thyroid hormone-responsive gene expression in rat liver via the increased number of nuclear receptors or changes in the chromatin constitution.

MeSH terms

  • Acetylation
  • Animals
  • Butyrates / pharmacology*
  • Butyric Acid
  • Cell Nucleus / chemistry*
  • Chromatin / metabolism
  • Gene Expression Regulation / drug effects*
  • Histones / biosynthesis
  • Histones / genetics
  • Liver / drug effects*
  • Liver / metabolism
  • Nuclear Proteins / biosynthesis*
  • Nuclear Proteins / genetics
  • Perfusion
  • Protein Processing, Post-Translational
  • Rats
  • Rats, Wistar
  • Receptors, Thyroid Hormone / biosynthesis*
  • Receptors, Thyroid Hormone / drug effects
  • Triiodothyronine / metabolism*
  • Up-Regulation


  • Butyrates
  • Chromatin
  • Histones
  • Nuclear Proteins
  • Receptors, Thyroid Hormone
  • Triiodothyronine
  • Butyric Acid