Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes

Biochem J. 1976 Oct 15;160(1):37-41. doi: 10.1042/bj1600037.

Abstract

Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34--36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.

MeSH terms

  • Acetylglucosamine / metabolism
  • Animals
  • Asparagine / metabolism*
  • Cattle
  • Cell Membrane / enzymology*
  • Glucosamine / metabolism
  • Manganese / pharmacology
  • Pancreas / enzymology
  • Ribonucleases / isolation & purification
  • Ribonucleases / metabolism*
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / ultrastructure

Substances

  • Manganese
  • Asparagine
  • Ribonucleases
  • Glucosamine
  • Acetylglucosamine