Abstract
The human F8/G253 antigen, B-CAM, is a cell surface glycoprotein that is expressed with restricted distribution pattern in normal fetal and adult tissues, and is up-regulated following malignant transformation in some cell types. We have isolated a complementary DNA for B-CAM using an expression cloning technique. The complementary DNA (EMBL accession number X80026) encodes a 588-amino acid protein which is a novel member of the immunoglobulin superfamily with a characteristic V-V-C2-C2-C2 immunoglobulin domain structure. This structure has been described previously for the human MUC18 melanoma antigen (31% amino acid identity) and chicken and rat versions of a neural adhesion molecule referred to as SC1/DM-GRASP/BEN or KG-CAM, respectively (26% amino acid identity). This homology is suggestive of a role for B-CAM in cell-cell or cell-matrix adhesion. The gene for B-CAM has been mapped by fluorescence in situ hybridization to chromosome 19q13.2-13.3.
MeSH terms
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Activated-Leukocyte Cell Adhesion Molecule
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Amino Acid Sequence
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Antigens, CD*
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Base Sequence
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Blotting, Northern
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CD146 Antigen
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Cell Adhesion Molecules / chemistry
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Cell Adhesion Molecules / genetics*
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Chromosomes, Human, Pair 19 / chemistry
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Cloning, Molecular
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Colonic Neoplasms / chemistry
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DNA, Complementary / chemistry
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DNA, Complementary / genetics*
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Extracellular Matrix Proteins / chemistry
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Extracellular Matrix Proteins / genetics
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Humans
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In Situ Hybridization, Fluorescence
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Lutheran Blood-Group System
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Membrane Glycoproteins / analysis
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics*
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Molecular Sequence Data
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Neoplasm Proteins / analysis
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / genetics*
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / genetics
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Neural Cell Adhesion Molecules*
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Tumor Cells, Cultured
Substances
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Activated-Leukocyte Cell Adhesion Molecule
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Antigens, CD
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BCAM protein, human
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CD146 Antigen
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Cell Adhesion Molecules
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DNA, Complementary
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Extracellular Matrix Proteins
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Lutheran Blood-Group System
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MCAM protein, human
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Membrane Glycoproteins
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Neoplasm Proteins
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Nerve Tissue Proteins
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Neural Cell Adhesion Molecules