Analysis of meiotic recombination events near a recombination hotspot in the yeast Saccharomyces cerevisiae

Curr Genet. 1994 Jul;26(1):21-30. doi: 10.1007/BF00326300.


The region of yeast chromosome III between the HIS4 and LEU2 genes has an unusually high frequency of meiotic recombination. In order to determine the pattern of cross-over and gene conversion events, we constructed a strain with a number of heterozygous markers in this 25-kb interval. We found that very high levels of recombination are localized to regions of DNA near HIS4. In addition, analysis of the patterns of co-conversion of adjacent markers suggests that there is more than one initiation site contributing to recombination of HIS4.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosomes, Fungal / metabolism*
  • Crossing Over, Genetic*
  • Gene Conversion
  • Genetic Complementation Test
  • Genetic Markers
  • Meiosis / genetics*
  • Saccharomyces cerevisiae / genetics*


  • Genetic Markers