Cyclin G is a transcriptional target of the p53 tumor suppressor protein

EMBO J. 1994 Oct 17;13(20):4816-22.

Abstract

Through a PCR-based differential screening method, cyclin G was identified as a novel transcriptional target of the p53 tumor suppressor gene product. In both a mouse p53 temperature-sensitive leukemic cell line and mouse embryonic fibroblasts (MEF) after gamma-irradiation, cyclin G mRNA was rapidly induced. MEF from a p53-deficient mouse expressed cyclin G at a level > 10-fold lower than that from a wild-type mouse. Using a DNA binding assay, a specific p53 binding site was identified upstream from the cyclin G gene, which functioned as a p53-dependent cis-acting element in a transient transfection assay. These results suggest that cyclin G might participate in a p53-mediated pathway to prevent tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cyclin G
  • Cyclin G1
  • Cyclins / biosynthesis
  • Cyclins / genetics*
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Transcription Factors / physiology*
  • Transcriptional Activation
  • Tumor Cells, Cultured
  • Tumor Suppressor Protein p53 / physiology*

Substances

  • Ccng1 protein, mouse
  • Cyclin G
  • Cyclin G1
  • Cyclins
  • Transcription Factors
  • Tumor Suppressor Protein p53

Associated data

  • GENBANK/Z37110