PAS7 encodes a novel yeast member of the WD-40 protein family essential for import of 3-oxoacyl-CoA thiolase, a PTS2-containing protein, into peroxisomes

EMBO J. 1994 Oct 17;13(20):4908-18.

Abstract

To identify components of the peroxisomal import pathway in yeast, we have isolated pas mutants affected in peroxisome biogenesis. Two mutants assigned to complementation group 7 define a new gene, PAS7, whose product is necessary for import of thiolase, a PTS2-containing protein, but not for that of SKL (PTS1)-containing proteins, into peroxisomes. We have cloned PAS7 by complementation of the oleic acid non-utilizing phenotype of the pas7-1 strain. The DNA sequence predicts a 42.3 kDa polypeptide of 375 amino acids encoding a novel member of the beta-transducin related (WD-40) protein family. A Myc epitope-tagged Pas7p, expressed under the control of the CUP1 promotor, was functionally active. Subcellular localization studies revealed that in the presence of thiolase this epitope-tagged Pas7p in part associates with peroxisomes. However, in a thiolase-deficient mutant, Pas7p was entirely found in the cytoplasm. We suggest that Pas7p mediates the binding of thiolase to these organelles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA C-Acyltransferase / metabolism*
  • Amino Acid Sequence
  • Base Sequence
  • Biological Transport / physiology
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / physiology
  • Cloning, Molecular
  • Fungal Proteins / genetics*
  • Fungal Proteins / physiology
  • Genes, Fungal*
  • Genetic Complementation Test
  • Microbodies / enzymology
  • Microbodies / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Peroxisomal Targeting Signal 2 Receptor
  • Receptors, Cytoplasmic and Nuclear*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins*

Substances

  • Cell Adhesion Molecules
  • Fungal Proteins
  • PEX7 protein, S cerevisiae
  • Peroxisomal Targeting Signal 2 Receptor
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae Proteins
  • Acetyl-CoA C-Acyltransferase

Associated data

  • GENBANK/X81424