The transcription factor, the Cdk, its cyclin and their regulator: directing the transcriptional response to a nutritional signal

EMBO J. 1994 Nov 15;13(22):5410-20.

Abstract

The Pho80-Pho85 cyclin-cdk complex prevents transcription of PHO5 by inhibiting the ability of the basic-helix-loop-helix transcription factor Pho4 to activate transcription in response to high phosphate conditions. In low phosphate the Pho80-Pho85 complex is inactivated and Pho4 is then able to activate the acid phosphatase gene PHO5. We show here that Pho4 and the homeobox protein Pho2 interact in vivo and act cooperatively to activate the PHO5 UAS, with interaction being regulated by the phosphate switch. In addition, we also demonstrate that an additional factor, Pho81, interacts in high phosphate with both the Pho80 cyclin and with Pho4. In low phosphate, Pho80 and Pho81 dissociate from Pho4, but retain the ability to interact with each other. The evidence presented here supports the idea that Pho81 acts as a phosphate-sensitive trigger that regulates the ability of the Pho80-Pho85 cyclin-cdk complex to bind Pho4, while DNA binding by Pho4 is dependent on the phosphate-sensitive interaction with Pho2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / biosynthesis*
  • Acid Phosphatase / genetics
  • Alkaline Phosphatase / biosynthesis
  • Alkaline Phosphatase / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Cell Cycle / physiology*
  • Cyclin-Dependent Kinases / physiology*
  • Cyclins*
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • DNA-Binding Proteins*
  • Enzyme Induction / drug effects
  • Fungal Proteins / physiology*
  • Gene Expression Regulation, Fungal / drug effects*
  • Helix-Loop-Helix Motifs
  • Homeodomain Proteins*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Phosphates / pharmacology
  • Protein Binding
  • Recombinant Fusion Proteins / metabolism
  • Repressor Proteins*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction*
  • Trans-Activators / physiology*
  • Transcription Factors*
  • Transcription, Genetic / drug effects

Substances

  • Cyclins
  • DNA, Fungal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Homeodomain Proteins
  • Macromolecular Substances
  • PHO2 protein, S cerevisiae
  • PHO4 protein, S cerevisiae
  • PHO80 protein, S cerevisiae
  • PHO81 protein, S cerevisiae
  • Phosphates
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Trans-Activators
  • Transcription Factors
  • Cyclin-Dependent Kinases
  • PHO85 protein, S cerevisiae
  • Alkaline Phosphatase
  • Acid Phosphatase