Involvement of phosphatidylinositol synthesis in the regulation of S phase induction

Exp Cell Res. 1994 Nov;215(1):228-33. doi: 10.1006/excr.1994.1336.

Abstract

The addition of serum to quiescent normal rat kidney (NRK) cells induced phosphatidylinositol (PI) synthesis after 4 h and DNA synthesis after 16 h. Inostamycin, an inhibitor of CDP-DG:inositol transferase, added within 4 h, inhibited both the serum-induced PI synthesis and S phase entry. By contrast, inostamycin added 8 h after release from quiescence did not inhibit the S phase entry. Inostamycin did not affect the G2/M/G1 transition, and incubation with inostamycin induced cell synchronization at early G1 phase. Thus, PI synthesis is involved in the regulation of S phase induction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Bombesin / pharmacology
  • Cell Cycle / drug effects
  • Cell Line
  • DNA / biosynthesis
  • DNA / drug effects
  • Epidermal Growth Factor / pharmacology
  • Furans / isolation & purification
  • Furans / pharmacology
  • Homeostasis
  • Inositol Phosphates / metabolism
  • Insulin / pharmacology
  • Kidney
  • Kinetics
  • Phosphatidylinositols / metabolism*
  • Platelet-Derived Growth Factor / pharmacology
  • Rats
  • S Phase / drug effects
  • S Phase / physiology*
  • Streptomyces
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Furans
  • Inositol Phosphates
  • Insulin
  • Phosphatidylinositols
  • Platelet-Derived Growth Factor
  • inostamycin
  • Epidermal Growth Factor
  • DNA
  • Bombesin