Decrease of heat shock protein 27/28 with heat stress in HTLV-I-transformed cells

Exp Mol Pathol. 1994 Jun;60(3):147-57. doi: 10.1006/exmp.1994.1014.

Abstract

We have previously reported an increase of human T-lymphotropic leukemia/lymphoma virus type I (HTLV-I) replication after heat treatment of MT-2 cells, an HTLV-I-transformed human lymphoid cell line. In this study, we investigated the effect of heat stress on the expression of Hsp27/28 in MT-2 cells. In contrast with previous studies of other cell types, a decrease of Hsp27/28 expression in MT-2 cells and an increase of Hsp70 family proteins in both MT-2 and uninfected lymphoid CEM cells were found following heat treatment at 42 degrees C. Furthermore, heat treatment resulted in an early rapid increase in the phosphorylated form of Hsp27/28 in both MT-2 and CEM cells. The results suggest that early post-translational phosphorylation of HSP27/28 could be a determinant of the ability of MT-2 cells to survive hyperthermia.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Northern
  • Blotting, Western
  • Cell Line, Transformed
  • Cell Transformation, Neoplastic / metabolism*
  • Cell Transformation, Viral / physiology*
  • Electrophoresis, Polyacrylamide Gel
  • Heat-Shock Proteins / metabolism*
  • Hot Temperature*
  • Human T-lymphotropic virus 1 / physiology*
  • Humans

Substances

  • Heat-Shock Proteins