Isolation and characterization of a mouse homolog of the Drosophila segment polarity gene dishevelled

Dev Biol. 1994 Nov;166(1):73-86. doi: 10.1006/dbio.1994.1297.

Abstract

In the Drosophila embryo dishevelled (dsh) function is required by target cells in order to respond to wingless (wg, the homolog of Wnt-1), demonstrating a role for dsh in Wnt signal transduction. We have isolated a mouse homolog of the Drosophila dsh segment polarity gene. The 695-amino-acid protein encoded by the mouse dishevelled gene (Dvl-1) shares 50% identity (65% similarity) with dsh. Similarity searches of protein and DNA data bases revealed that Dvl-1 encodes an otherwise novel polypeptide. While no functional motifs were identified, one region of Dvl-1 was found to be similar to a domain of discs large-1 (dlg), a Drosophila tumor suppressor gene. In the embryo, Dvl-1 is expressed in most tissues, with uniformly high levels in the central nervous system. From 7.5 days postcoitum Dvl-1 is expressed throughout the developing brain and spinal cord, including those regions expressing Wnt-1 and En. Expression of Dvl-1 in adult mice was found to be widespread, with brain and testis exhibiting the highest levels. The majority of Dvl-1 expression in the adult cerebellum is in the granular cell layer, similar to the pattern seen for engrailed-2 (En-2). Throughout postnatal development of the brain Dvl-1 is highly expressed in areas of high neuronal cell density.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Aging / metabolism
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Brain / growth & development
  • Brain / metabolism
  • DNA / chemistry
  • DNA Primers
  • Dishevelled Proteins
  • Drosophila / enzymology
  • Drosophila / genetics*
  • Drosophila Proteins
  • Embryo, Nonmammalian / physiology
  • Embryonic and Fetal Development
  • Gene Expression*
  • Gestational Age
  • Humans
  • Male
  • Mice / genetics*
  • Molecular Sequence Data
  • Organ Specificity
  • Phosphoproteins*
  • Polymerase Chain Reaction
  • Protein Biosynthesis*
  • Proteins / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Species Specificity
  • Spinal Cord / embryology
  • Spinal Cord / metabolism
  • Testis / embryology
  • Testis / growth & development
  • Testis / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • DNA Primers
  • DVL1 protein, human
  • Dishevelled Proteins
  • Drosophila Proteins
  • Dvl1 protein, mouse
  • Phosphoproteins
  • Proteins
  • RNA, Messenger
  • dsh protein, Drosophila
  • DNA

Associated data

  • GENBANK/U10115