Chondroitin Sulfate Proteoglycan Elevates Cytoplasmic Calcium in DRG Neurons

Dev Biol. 1994 Nov;166(1):87-100. doi: 10.1006/dbio.1994.1298.

Abstract

Proteoglycans have been implicated in neuronal path-finding during development, yet related second messenger and signaling systems are unknown. We have used the calcium indicator fura-2/AM to monitor cytoplasmic calcium ion concentration ([Ca2+]i) in chick dorsal root ganglion (DRG) neuronal growth cones elongating on laminin during contact with chondroitin sulfate proteoglycan (CSPG): (1) to determine whether there is a change in [Ca2+]i in neurons that contact CSPG, and (2) to determine whether changes in [Ca2+]i are necessary for inhibition of growth cone migration. The majority of DRG neurons responded to CSPG contact with a transient rise in [Ca2+]i (mean delta[Ca2+]i above resting level was 554 +/- 109 nM; P < 0.0001). The effect of CSPG contact was concentration dependent and required the carbohydrate moiety of CSPG. Addition of soluble CSPG did not elevate [Ca2+]i. Treatment with reagents that blocked plasma membrane calcium channels, or that perturbed intracellular Ca2+ stores, indicated that extracellular Ca2+ was the major source of the [Ca2+]i elevation, and that Ca2+ entry occurred through non-voltage-gated calcium channels. Although general Ca2+ channel blockers abolished the CSPG-induced [Ca2+]i rise, they did not abolish growth cone avoidance of surface-bound CSPG in these assays. We conclude: (1) that DRG neurons elevate [Ca2+]i in response to CSPG contact to levels that can modify cytoskeletal mechanisms of growth cone migration, and (2) that avoidance of substratum-bound CSPG may not be dependent upon elevated [Ca2+]i.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Calcium Channel Blockers / pharmacology*
  • Calcium Channels / drug effects
  • Calcium Channels / metabolism*
  • Chick Embryo
  • Chondroitin Sulfate Proteoglycans / pharmacology*
  • Ganglia, Spinal / cytology
  • Ganglia, Spinal / metabolism*
  • Kinetics
  • Laminin
  • Microscopy, Phase-Contrast
  • Neurons / cytology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Time Factors

Substances

  • Calcium Channel Blockers
  • Calcium Channels
  • Chondroitin Sulfate Proteoglycans
  • Laminin
  • Calcium