Polymerase chain reaction mediated localization of RFLP clones to microisolated translocation chromosomes of barley

Genome. 1994 Aug;37(4):550-5. doi: 10.1139/g94-078.


A new strategy has been devised and used for the physical localization of genetically mapped restriction fragment length polymorphism (RFLP) clones to barley chromosomes. Morphologically distinct translocation chromosomes from synchronized root-tip meristems were microisolated and their DNA was used as a template for polymerase chain reaction with sequence-specific primers. Four RFLP clones were assigned to cytologically defined segments of chromosome 5. This related approximately one-third of the map length of linkage group 5 to approximately one-fifth of the mitotic metaphase length of chromosome 5. The technique may substantially contribute to the connection of the RFLP-based genetic linkage maps with cytological markers of the barley chromosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Chromosomes
  • Cloning, Molecular
  • DNA Primers / genetics
  • Genetic Linkage
  • Hordeum / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Recombination, Genetic
  • Restriction Mapping
  • Translocation, Genetic


  • DNA Primers