A multifunctional vector family for gene expression in mammalian cells

Gene. 1994 Nov 18;149(2):387-8. doi: 10.1016/0378-1119(94)90186-4.


A family of different promoters and vector backbones for multiple use in mammalian cells in vivo and in vitro experiments has been created. The elements allow high-level constitutive, as well as adjustable expression with mono- and dicistronic transcription units and several in vitro manipulations of the inserted ORFs. The vector components are designed to be combined in a simple cloning step.

MeSH terms

  • Animals
  • Cell Line
  • Cloning, Molecular / methods
  • Gene Expression*
  • Genes*
  • Genetic Vectors*
  • Mammals
  • Open Reading Frames
  • Promoter Regions, Genetic*
  • Protein Biosynthesis
  • Transcription, Genetic
  • Transfection / methods*
  • Xenopus laevis