Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII

M Rina; M Markaki; V Bouriotis

doi:10.1016/0378-1119(94)90860-5

Sequence of the cloned bseCIM gene: M.BseCI reveals high homology to M.BanIII

Gene. 1994 Dec 2;150(1):71-3. doi: 10.1016/0378-1119(94)90860-5.

Authors

M Rina¹, M Markaki, V Bouriotis

Affiliation

¹ Institute of Molecular Biology and Biotechnology, Enzyme Technology Division, Crete, Greece.

PMID: 7959066
DOI: 10.1016/0378-1119(94)90860-5

Abstract

The bseCIM gene, encoding M.BseCI methyltransferase (MTase) from a Bacillus stearothermophilus strain, has been previously cloned and expressed in Escherichia coli [Rina and Bouriotis, Gene 133 (1993) 91-94]. The nucleotide (nt) sequence of a 2357-bp BspMII-EcoRI fragment encoding bseCIM has now been determined. The sequence predicts a MTase of 579 amino acids (aa), 66.7 kDa. Comparison of the deduced aa sequence of M.BseCI with sequences of various MTases revealed a significant homology to m6A-MTases, especially to its isoschizomer M.BanIII from Bacillus aneurinolyticus.

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Geobacillus stearothermophilus / enzymology
Geobacillus stearothermophilus / genetics
Molecular Sequence Data
Sequence Homology, Amino Acid
Site-Specific DNA-Methyltransferase (Adenine-Specific) / genetics*

Substances

DNA modification methylase Cla I
Site-Specific DNA-Methyltransferase (Adenine-Specific)

Associated data

GENBANK/X79509