A nitric oxide synthase inhibitor reduces inflammation, down-regulates inflammatory cytokines and enhances interleukin-10 production in carrageenin-induced oedema in mice

Immunology. 1994 Jul;82(3):370-5.


Mice injected in the footpad with carrageenin developed local inflammation which peaked at 48 hr. This delayed-type footpad swelling was significantly reduced in mice injected intraperitoneally (i.p.) with a specific nitric oxide (NO) synthase inhibitor, L-NGmonomethyl-arginine (L-NMMA). The draining lymph node (DLN) cells from mice injected 48 hr previously with carrageenin produced significantly higher levels of proliferation and interleukin-1 (IL-1), IL-2, IL-6 and interferon-gamma (IFN-gamma), but less IL-10, compared to cells from saline-injected controls, when stimulated with concanavalin A (Con A) in vitro. Treatment of the carrageenin-injected mice with L-NMMA had little effect on the proliferative response of the DLN cells, but significantly reduced the production of IL-1, IL-2, IL-6 and IFN-gamma, and increased the secretion of IL-10. These data demonstrate that NO plays a significant role in local inflammation and the pattern of cytokines induced in this model.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / analogs & derivatives
  • Arginine / therapeutic use
  • Carrageenan
  • Down-Regulation
  • Drug Hypersensitivity / etiology
  • Drug Hypersensitivity / prevention & control
  • Edema / chemically induced
  • Edema / immunology*
  • Edema / prevention & control
  • Female
  • Hypersensitivity, Delayed / chemically induced
  • Hypersensitivity, Delayed / immunology*
  • Hypersensitivity, Delayed / prevention & control
  • Inflammation / prevention & control*
  • Interferon-gamma / biosynthesis*
  • Interleukin-10 / biosynthesis
  • Interleukins / biosynthesis*
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / physiology*
  • omega-N-Methylarginine


  • Interleukins
  • Interleukin-10
  • omega-N-Methylarginine
  • Nitric Oxide
  • Interferon-gamma
  • Carrageenan
  • Arginine