Effect of cell growth rate on expression of the anaerobic respiratory pathway operons frdABCD, dmsABC, and narGHJI of Escherichia coli

J Bacteriol. 1994 Nov;176(21):6599-605. doi: 10.1128/jb.176.21.6599-6605.1994.

Abstract

The fumarate reductase (frdABCD), dimethyl sulfoxide (DMSO)-trimethylamine-N-oxide (TMAO) reductase (dmsABC), and nitrate reductase (narGHJI) operons in Escherichia coli encode enzymes involved in anaerobic respiration to the electron acceptors fumarate, DMSO or TMAO, and nitrate, respectively. They are regulated in response to anaerobiosis and nitrate availability. To determine how each operon is regulated in response to changes in cell growth rate and in oxygen availability, expression of frdA-lacZ, dmsA-lacZ, and narG-lacZ fusion genes was examined during continuous culture. After a change in the cell growth rate, each anaerobic electron transport pathway operon fusion responded somewhat differently. Whereas frdA-lacZ expression increased by fivefold as the growth rate decreased from 0.60 to 0.12/hour during aerobic growth, little change was seen under anaerobic conditions. In contrast, growth rate-dependent expression of narG-lacZ expression occurred under anaerobic conditions but not under aerobic conditions. Finally, dmsA-lacZ expression did not vary greatly for any of the growth rates tested. When cells were shifted from aerobic to anaerobic growth conditions, expression of each fusion increased at a moderate rate and peaked or "overshot" before reaching a new equilibrium value. This "overshoot" phenomenon was independent of the fnr gene product, which functions as a transcriptional activator of each respiratory operon during anaerobic conditions. In contrast to the moderate rate of anaerobic induction seen for narG-lacZ expression, the addition of nitrate caused a rapid induction response. The cell appears to have many ways to adjust cell respiration in response to changes in cell growth conditions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aerobiosis
  • Anaerobiosis
  • Cell Division
  • Electron Transport / genetics*
  • Escherichia coli / genetics
  • Escherichia coli / growth & development*
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Bacterial* / drug effects
  • Genes, Reporter
  • Iron-Sulfur Proteins*
  • Lac Operon
  • Nitrates / pharmacology
  • Nitrite Reductases / biosynthesis
  • Nitrite Reductases / genetics
  • Operon / genetics
  • Oxidoreductases / biosynthesis*
  • Oxidoreductases / genetics
  • Oxidoreductases, N-Demethylating / biosynthesis
  • Oxidoreductases, N-Demethylating / genetics
  • Oxygen / pharmacology
  • Recombinant Fusion Proteins / biosynthesis
  • Succinate Dehydrogenase / biosynthesis
  • Succinate Dehydrogenase / genetics

Substances

  • Iron-Sulfur Proteins
  • Nitrates
  • Recombinant Fusion Proteins
  • Oxidoreductases
  • Succinate Dehydrogenase
  • Oxidoreductases, N-Demethylating
  • trimethylamine dehydrogenase
  • Nitrite Reductases
  • dimethyl sulfoxide reductase
  • Oxygen