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Comparative Study
, 127 (3), 581-92

Human CENP-A Contains a Histone H3 Related Histone Fold Domain That Is Required for Targeting to the Centromere

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Comparative Study

Human CENP-A Contains a Histone H3 Related Histone Fold Domain That Is Required for Targeting to the Centromere

K F Sullivan et al. J Cell Biol.

Abstract

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. To examine the molecular basis for the specification of centromeric chromatin, we have cloned a human cDNA that encodes the 17-kD histone-like centromere antigen, CENP-A. Two domains are evident in the 140 aa CENP-A polypeptide: a unique NH2-terminal domain and a 93-amino acid COOH-terminal domain that shares 62% identity with nucleosomal core protein, histone H3. An epitope tagged derivative of CENP-A was faithfully targeted to centromeres when expressed in a variety of animal cells and this targeting activity was shown to reside in the histone-like COOH-terminal domain of CENP-A. These data clearly indicate that the assembly of centromeres is driven, at least in part, by the incorporation of a novel core histone into centromeric chromatin.

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