An Hsp60 related protein is associated with purified HIV and SIV

J Med Primatol. 1994 Feb-May;23(2-3):151-4. doi: 10.1111/j.1600-0684.1994.tb00116.x.

Abstract

The possible physical association of heat shock proteins (Hsp's) with immunodeficiency viruses (HIV and SIV) has been examined. The virions were purified by a) polyethylene glycol (PEG) precipitation and Sepharose 4B filtration, b) PEG precipitation and centrifugation over a Renografin gradient, or c) PEG precipitation and Matrex Cellufine Sulfate affinity chromatography. Western blotting revealed an Hsp60 related protein associated with HIV and SIV. Other Hsp's (such as Hsp70) were not detected, suggesting a specific interaction between Hsp60 and viral factors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Blotting, Western
  • Cell Line
  • Chaperonin 60 / isolation & purification
  • Chaperonin 60 / metabolism*
  • Chromatography, Affinity
  • Electrophoresis, Polyacrylamide Gel
  • HIV / isolation & purification
  • HIV / metabolism*
  • Humans
  • Polyethylene Glycols
  • Protein Binding
  • Simian Immunodeficiency Virus / isolation & purification
  • Simian Immunodeficiency Virus / metabolism*
  • T-Lymphocytes
  • Virion / isolation & purification
  • Virion / metabolism

Substances

  • Antibodies, Monoclonal
  • Chaperonin 60
  • Polyethylene Glycols